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od 2020-09-20

Title: Crystallization and preliminary crystallographic studies of five crystal forms of Escherichia Coli L-asparaginase II (Asp90Glu mutant)

Source: Acta Crystallographica Section D-Biological Crystallography

Year : 2002

Abstract:

L-Asparaginase II from Escherichia coli with an Asp90Glu mutation in the active site has been crystallized in five polymorphic forms. Crystals of all polymorphs suitable for X-ray diffraction experiments were obtained by the vapour-diffusion method. Crystals of form I belong to the monoclinic system (space group C2), have unit-cell parameters a = 73.1, b = 133.1, c = 62.6 Angstrom, beta = 108.8 degrees and diffract to 2.27 degrees resolution. Three of the crystal forms are orthorhombic, with unit-cell parameters a = 225.4, b = 128.0, c = 62.6 Angstrom (form II, P2(1)2(1)2), a = 59.9, b = 71.2, c = 130.6 Angstrom (form III, primitive cell) and a = 73.8, b = 122.1, c = 124.2 Angstrom (form IV, P2(1)2(1)2(1) or P2(1)2(1)2); the crystals diffract to 2.33, 3.5 and 1.7 Angstrom, respectively. Polymorph V is trigonal, space group P3(1)21, with unit-cell parameters a = 123.1, c = 83.8 Angstrom; the crystals diffract to 2.65 Angstrom resolution.

DOI: 10.1107/S0907444901016663   (Pobrane:  2020-10-23)