wizyta

od 2020-09-20

Title: The influence of radiation sterilization on thiamphenicol

Source: Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy

Year : 2008

Abstract:

The effect of ionising radiation, applied in the form of an electron beam, in the doses of 25, 100 and 400kGy on the physical and chemical properties of thiamphenicol in solid phase has been studied by organoleptic analysis (form, colour, smell, solubility, clarity) and spectroscopic methods (UV, IR, EPR), chromatography (TLC), SEM observations, X-ray diffraction, polarimetry and thermal method (DSC).
The above-discussed results have proved that on irradiation with a dose of 25 kGy no significant changes appear in thiamphenicol, apart from the formation of free radicals of the lifetime of over 352 days. On irradiation with much higher doses (100 and 400 kGy) no changes were observed in the IR spectra but the UV line intensities slightly increased at lambda(max) = 266 and 273 nm, the colour of the powder changed, the radiolysis products appeared as detected by TLC, changes were also observed in the XRD, SEM pictures, the melting point values (DSC) and optical rotation.
On the basis of DSC results a linear relation was found between the irradiation dose and the decrease in the melting point and increase in the enthalpy of melting, characterised by high correlation coefficients of r = 0.9839 and 0.9622, respectively. Moreover, a linear relation was established between the optical rotation angle and the irradiation dose, alpha(D) (degrees) =f(dose), characterised by the correlation coefficient r = 0.9874.
The results obtained indicate that thiamphenicol can be safely subjected to radiation sterilization by the standard dose of 25 kGy.
(C) 2007 Elsevier B.V. All rights reserved.

DOI: 10.1016/j.saa.2007.05.038   (Pobrane:  2020-10-21)