wizyta

od 2020-09-20

Title: Studies of zwitterionic lipoplexes - nanosystem based on phospholipids and surfactants as innovative delivery systems for gene therapy

Source: Biophysical Journal

Year : 2015

Abstract:

The gene therapy is the one of the most promising method of treatment in contemporary medicine. This way of therapy is very useful in the treatment a dozen of incurable or fatal diseases. This method of treatment is leaned on implement a gene into patient's cells with the use of dedicated delivery systems (vectors). The main problem of gene therapy is to find the best vector which will be effective and will be not toxic for human cells. A good approach seems to be use of non-viral vectors like delivery system based on lipid-surfactant mixtures.

The aim of this study was to examine the possible application of selected amphoteric surfactants (zwitterionic alkyl derivatives of sulfobetaine) as complexing agents (with and without helper lipid) for nucleic acids (siRNA, low and high-molecular weight DNA).

The studies of DNA conformation in selected DNA - zwitterionic surfactant lipoplexes were performed using the circular dichroism (CD) spectroscopy. CD spectra were recorded in the spectral range 350 - 200 nm by using J-815 spectrometer (Jasco). The results obtained indicate that the DNA maintains the B-form for wide range of surfactant concentrations in the solution. The structure and organization of lipoplexes was also independently analyzed by the Fourier transform infrared spectroscopy. The absorption spectra for lipoplexes were collected by using FTIR spectrometer BRUKER Tensor 27 in the temperature range 2-40°C. The phase transitions in examined systems were studied by using the differential scanning calorimetry (DSC). Ability of creating of stable complexes in DNA-surfactant systems studied was confirmed using electrophoresis on agarose gel. For all formed stable lipoplexes the complete reduction of electrophoretic mobility was observed. Finally the transfection efficiency of selected systems were also tested on HeLa cells.

DOI: 10.1016/j.bpj.2014.11.2989   (Pobrane:  2020-11-05)