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od 2020-09-20

Title: PEG-MWCNT/Fe hybrids as multi-modal contrastagents for MRI and optical imaging

Source: RSC Advances

Year : 2016

Abstract:

This study examines the use of oxidized multi-walled carbon nanotube/iron (O-MWCNT/Fe) nanohybrids modified with polyethylene glycol (PEG) as multifunctional cellular imaging agents for magnetic resonance imaging (MRI) and fluorescence microscopy. The PEGylated MWCNTs with embedded iron particles were investigated as T2-weighted contrast agents for MRI. The number of PEG molecules attached to the MWCNT surface was calculated. The PEG-MWCNT/Fe complex was characterized by transmission electron microscopy, scanning electron microscopy, Fourier transform infrared and Raman spectroscopies. Covalent surface modification of the MWCNTs improves their solubility and enables the attachment of further biomolecules to their surface. The PEGylated nanostructures were labeled with the MDC organic dye and internalized inside HeLa cells for cellular imaging. Additionally, the minima cytotoxic effect of PEGylated complexes in comparison to non-PEGylated samples was measured Rusing the WST-1 test and an In Cell Analyzer. A confocal microscopy study of the organelle morphology also confi rmed that the HeLa cell morphology was unchanged after treatment with PEGylated MWCs.

DOI: 10.1039/c6ra09191a   (Pobrane:  2018-04-04)