wizyta

od 2020-09-20

Title: Crystallization and preliminary crystallographic studies of a new crystal form of papain from Carica papaya

Source: Acta Biochimica Polonica

Year : 1997

Abstract:

A new crystal form of papain from the latex of Carica papaya, complexed with an inhibitor (Z-Arg-Leu-Val-Gly-CHN2) was obtained by the vapor-diffusion method using a methanol/ethanol mixture as a precipitant. The slat-like crystals are monoclinic, space group P2(1), with unit cell parameters a = 42.6 Angstrom, b = 49.8 Angstrom, c = 50.5 Angstrom, beta = 111.9 degrees, and contain one molecule in the asymmetric unit. The crystals are stable in the X-ray beam and diffract beyond 1.8 Angstrom. A molecular model has been placed in the unit cell by molecular replacement.

   (Pobrane:  aktualizowanie)