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od 2020-09-20

Dr inż. Barbara Maciejewska  | 2010-10 <> 2016-10

Stażysta podoktorski

   CNBM UAM      +48 61-829-6710       maciejewska.barbara@gmail.com

  0000-0002-3101-366X     55991083000  

Zainteresowania naukowe:
Synteza nanostruktur węglowych przy użyciu metody Chemicznego Osadzania z Fazy Gazowej (CVD).
Modyfikacja powierzchni jednościennych nanorurek węglowych.
Środki kontrastujące w obrazowaniu NMR.

Publikacje      Projekty           Magistrowie           Seminaria


14.

Maciejewska B.M., Wychowaniec J.K., Woźniak-Budych M., Popenda Ł., Warowicka A., Golba K., Litowczenko J., Fojud Z., Wereszczyńska B., Jurga S.

UV cross-linked polyvinylpyrrolidone electrospun fibres as antibacterial surfaces Many bacteria become progressively more resistant to antibiotics and it remains a challenging task to control their overall levels. Polymers combined with active biomolecules come to the forefront for the design of antibacterial materials that can address this encounter. In this work, we investigated the photo-crosslinking approach of UV-sensitive benzophenone molecule (BP) with polyvinylpyrrolidone (PVP) polymer within electrospun fibres. The BP and PVP solutions allowed fabricating polymer mats that were subsequently functionalised with antibacterial lysozyme. The physical properties of the crosslinked electrospun fibres were investigated by scanning electron microscopy and atomic force microscopy. The average diameter of the obtained fibres decreased from 290 ± 50 nm to 270 ± 70 nm upon the addition of the crosslinking molecules and then to 240 ± 80 nm and 180 ± 90 nm after subsequent crosslinking reaction at an increasing time: 3 and 5 h, respectively. The peak force quantitative nanomechanical mapping (PF-QNM) indicated the increase of DMT modulus of obtained cross-linked fibres from 4.1 ± 0.8 GPa to 7.2 ± 0.5 GPa. Furthermore, the successful crosslinking reaction of PVP and BP solution into hydrogels was investigated in terms of examining photo-crosslinking mechanism and was confirmed by rheology, Raman, Fourier transform infrared and nuclear magnetic resonance. Finally, lysozyme was successfully encapsulated within cross-linked PVP-BP hydrogels and these were successfully electrospun into mats which were found to be as effective antibacterial agents as pure lysozyme molecules. The dissolution rate of photo cross-linked PVP mats was observed to increase in comparison to pure PVP electrospun mats which opened a potential route for their use as antibacterial, on-demand, dissolvable coatings for various biomedical applications.

Science and Technology of Advanced Materials, 20(1), 979-991 (2019)

DOI: 10.1080/14686996.2019.1667737   (Pobrane:  2020-06-04)


13.

Litowczenko J., Maciejewska B.M., Wychowaniec J.K., Kościnski M., Jurga S., Warowicka A.

Groove-patterned surfaces induce morphological changes in cells of neuronal origin It is already known that cells respond strongly to topography and chemistry of 2D surfaces. In this work we study cell-material interactions; in particular, we investigated the attachment and alignment of SH-SY5Y cells of neuronal origin on grooved-patterns made from Silicon (Si) and Gold (Au). The Au-Si groove-pattern stimulated 93% of SH-SY5Y cells to differentiate into neuroblast-like type (N-type) in 2 days and outgrown neurites exhibited strong anisotropy along the grooves with 90% of cells having one or two neurites. In comparison, random distribution of morphology type, neurite number, and alignment were observed on control flat surfaces (Si and Au). We further show that designed Au-Si groove-patterns can be used to form reversed groove patterns on polycarolactone surface via soft lithography approach. Sixty-nine percentage of SH-SY5Y cells aligned along the obtained reversed groove patterns of the same dimensional characteristics to Si-Au grooves. In particular, this work demonstrated that the Au-Si grooves pattern stimulates neurite polarity, elongation, and morphological differentiation of neuroblastoma cells without any exogenous supply of growth factors or stimulants in just 2 days, which can lead to selective procedure of obtaining homologous population of neuron-like cells for future nerve regeneration therapies.

Journal of Biomedical Materials Research part A, 107(10), 2244-2256 (2019)

DOI: 10.1002/jbm.a.36733   (Pobrane:  2020-12-30)


12.

Rychter M., Baranowska-Korczyc A., Milanowski B., Jarek M., Maciejewska B.M., Coy E.L., Lulek J.

Cilostazol-loaded poly(epsilon-caprolactone) electrospun drug delivery system for cardiovascular applications Purpose The study discusses the value of electrospun cilostazol-loaded (CIL) polymer structures for potential vascular implant applications. Methods Biodegradable polycaprolactone (PCL) fibers were produced by electrospinning on a rotating drum collector. Three different concentrations of CIL: 6.25%, 12.50% and 18.75% based on the amount of polymer, were incorporated into the fibers. The fibers were characterized by their size, shape and orientation. Materials characterization was carried out by Fourier Transformed Infrared spectroscopy (FTIR), Raman spectroscopy, differential scanning calorimetry (DSC) and X-ray diffraction (XRD). In vitro drug release study was conducted using flow-through cell apparatus (USP 4). Results Three-dimensional structures characterized by fibers diameter ranging from 0.81 to 2.48 mu m were in the range required for cardiovascular application. DSC and XRD confirmed the presence of CIL in the electrospun fibers. FTIR and Raman spectra confirmed CIL polymorphic form. Elastic modulus values for PCL and the CIL-loaded PCL fibers were in the range from 0.6 to 1.1 GPa. The in vitro release studies were conducted and revealed drug dissolution in combination with diffusion and polymer relaxation as mechanisms for CIL release from the polymer matrix. Conclusions The release profile of CIL and nanomechanical properties of all formulations of PCL fibers demonstrate that the cilostazol loaded PCL fibers are an efficient delivery system for vascular implant application.

Pharmaceutical Research, 35(2), UNSP 32 (2018)

DOI: 10.1007/s11095-017-2314-0   (Pobrane:  2019-03-20)


11.

Woźniak-Budych M.J., Przysiecka Ł., Maciejewska B.M., Wieczorek D., Staszak K., Jarek M., Jesionowski T., Jurga S.

Facile synthesis of sulfobetaine-stabilized Cu2O nanoparticles and their biomedical potential A novel approach using a zwitterionic sulfobetaine-based surfactant for the synthesis of spherical copper oxide nanoparticles (Cu2O NPs) has been applied. For the first time, N-hexadecyl-N,N-dimethyl-3-ammonio-1-propanesulfonate has been used as stabilizer to control the size and morphology of Cu2O NPs. Several techniques, such as transmission electron microscopy (TEM), X-ray diffraction (XRD), and fluorescence spectroscopy, are used to investigate the size, structure, and optical properties of synthesized Cu2O nanocrystals. The results indicate that copper(I) oxide nanoparticles with size in the range of 2 to 45 nm and crystalline structure, exhibit intense yellow fluorescence (lambda(em) = 575 nm). Furthermore, the cytotoxicity studies show that sulfobetaine-stabilized copper oxide nanoparticles prompt inhibition of cancer cell proliferation in a concentration-dependent manner, however, the adverse effect on the normal cells has also been observed. The results indicate that the sulfobetaine-stabilized Cu2O, because of their unique properties, have a potential to be applied in medical fields, such as cancer therapy and bioimaging.

ACS Biomaterials Science & Engineering, 3(12), 3183-3194 (2017)

DOI: 10.1021/acsbiomaterials.7b00465   (Pobrane:  2018-03-20)


10.

Warowicka A., Maciejewska B.M., Litowczenko J., Kościński M., Baranowska-Korczyc A., Jasiurkowska-Delaporte M., Kozioł K., Jurga S.

Abstrakt lub materiały konferencyjne, albo abstrakt publikacji
z dodatkowymi numerami DOI

Corrigendum to “MWCNT based matrices as a platform for adhesion and growth of cells” [Compos. Sci. Technol. 136 (2016) 29-38] The authors regret that the author Anna Baranowka-Korczyc is actually Anna Baranowska-Korczyc.

The authors would like to apologise for any inconvenience caused.

Composites Science and Technology, 149, 305-305 (2017)

DOI: 10.1016/j.compscitech.2017.05.023   (Pobrane:  2018-03-20)


9.

Jaroszewski K., Chrunik M., Głuchowski P.Coy E., Maciejewska B., Jastrząb R., Majchrowski A., Kasprowicz D.

Photoluminescence properties of Pr3+ doped Bi2ZnOB2O6 microcrystals and PMMA-based composites Photoluminescence properties of red-emitting Pr3+-doped Bi2ZnOB2O6 microcrystalline powder and PMMA-based composite materials were reported. Bi2ZnOB2O6:Pr3+ powders were synthesized by means of the modified Pechini method. The morphology and crystallographic structure of Bi2ZnOB2O6:Pr3+ microcrystals were investigated by XRD and HRTEM. The PMMA-based composite materials were prepared by embedding of Bi2ZnOB2O6:Pr3+ powder in the PMMA matrix. The vibrational properties of the powder and composite systems were investigated by µ-Raman spectroscopy. Emission spectra of the samples were measured under blue (451.6 nm) and UV (320 nm) excitation. Both, the powder and composite samples show enhancement of red emission (1D2 › 3H4) and quenching of greenish-blue luminescence from 3P0 level of Pr3+ ions after excitation in UV and VIS caused by the 3P0 ~ 1D2 non-radiative relaxation of Pr3+ ion by low-lying charge transfer state and non-radiative de-excitation through Pr3+-Bi3+ energy transfer. The response of the powder and composite samples to pulsed excitation at 451.6 nm was measured by monitoring emission from the 1D2 level (595 nm/3P0 › 3H6 transition). The determined lifetimes ?1 and ?2 of the red emission at 595 nm (1D2 › 3H4 transition) of the composite are significantly longer in comparison to powder samples. Moreover, because of the good nonlinear optical properties of the Bi2ZnOB2O6 crystals and effective luminescence of the Pr3+-doped Bi2ZnOB2O6 powders and composites, they can be very useful as bi-functional materials in the new generation of optoelectronic devices.

Optical Materials, 62, 72-79 (2016)

DOI: 10.1016/j.optmat.2016.09.059


8.

Warowicka A., Maciejewska B.M., Litowczenko J., Kościński M., Baranowska-Korczyc A., Jasiurkowska-Delaporte M., Kozioł K., Jurga S.

MWCNT based matrices as a platform for adhesion and growth of cells In this study we examine multiwall carbon nanotube (MWCNT) based nanosystems i.e. aligned MWCNT matrices, as potential scaffolds for cell growth and tissue engineering. We present the effect of samples purification and surface modification on the viability, adhesiveness and morphology of human normal gingival fibroblasts (HGF-1) and human osteosarcoma (U2OS) cell lines. We employ spectroscopic and analytical techniques (Scanning Electron Microscopy, Thermal Gravimetric Analysis, Atomic Force Microscopy, Raman and Fourier Transform Infrared Absorption Spectroscopy and Confocal Microscopy) in order to characterize the obtained structures. Furthermore, we analyze the expression level of selected cell adhesion-related genes by the quantitative real-time (qRT-PCR) method and the cell viability for MWCNT powders. We show that the surface structure of MWCNTs carpets contributes to the adhesion of cells. Additionally, we report the increased expression level of integrin, talin and fibronectin, proteins which are involved in cell attachment. We speculate that carbon nanotube based materials can be consider as potential candidates for biomedical purposes and as a biocompatible scaffold for cell growth.

Composites Science and Technology, 136, 29-38 (2016)

DOI: 10.1016/j.compscitech.2016.09.026   (Pobrane:  2018-03-19)


7.

Jaroszewski K., Chrunik M., Głuchowski P., Coy E., Maciejewska B., Jastrząb R., Majchrowski A., Kasprowicz D.

Photoluminescence properties of Pr3+ doped Bi2ZnOB2O6 microcrystals and PMMA-based composites Photoluminescence properties of red-emitting Pr3+-doped Bi2ZnOB2O6 microcrystalline powder and PMMA-based composite materials were reported. Bi2ZnOB2O6:Pr3+ powders were synthesized by means of the modified Pechini method. The morphology and crystallographic structure of Bi2ZnOB2O6:Pr3+ microcrystals were investigated by XRD and HRTEM. The PMMA-based composite materials were prepared by embedding of Bi2ZnOB2O6:Pr3+ powder in the PMMA matrix. The vibrational properties of the powder and composite systems were investigated by μ-Raman spectroscopy. Emission spectra of the samples were measured under blue (451.6 nm) and UV (320 nm) excitation. Both, the powder and composite samples show enhancement of red emission (1D2 → 3H4) and quenching of greenish-blue luminescence from 3P0 level of Pr3+ ions after excitation in UV and VIS caused by the 3P0 ∼ 1D2 non-radiative relaxation of Pr3+ ion by low-lying charge transfer state and non-radiative de-excitation through Pr3+–Bi3+ energy transfer. The response of the powder and composite samples to pulsed excitation at 451.6 nm was measured by monitoring emission from the 1D2 level (595 nm/3P0 → 3H6 transition). The determined lifetimes τ1 and τ2 of the red emission at 595 nm (1D2 → 3H4 transition) of the composite are significantly longer in comparison to powder samples. Moreover, because of the good nonlinear optical properties of the Bi2ZnOB2O6 crystals and effective luminescence of the Pr3+-doped Bi2ZnOB2O6 powders and composites, they can be very useful as bi-functional materials in the new generation of optoelectronic devices.

Optical Materials, 62, 72-79 (2016)

DOI: 10.1016/j.optmat.2016.09.059   (Pobrane:  2020-10-21)


6.

Baranowska-Korczyc A., Jasiurkowska-Delaporte M., Maciejewska B.M., Warowicka A., Coy L.E., Zalewski T., Kozioł K.K., Jurga S.

PEG-MWCNT/Fe hybrids as multi-modal contrastagents for MRI and optical imaging This study examines the use of oxidized multi-walled carbon nanotube/iron (O-MWCNT/Fe) nanohybrids modified with polyethylene glycol (PEG) as multifunctional cellular imaging agents for magnetic resonance imaging (MRI) and fluorescence microscopy. The PEGylated MWCNTs with embedded iron particles were investigated as T2-weighted contrast agents for MRI. The number of PEG molecules attached to the MWCNT surface was calculated. The PEG-MWCNT/Fe complex was characterized by transmission electron microscopy, scanning electron microscopy, Fourier transform infrared and Raman spectroscopies. Covalent surface modification of the MWCNTs improves their solubility and enables the attachment of further biomolecules to their surface. The PEGylated nanostructures were labeled with the MDC organic dye and internalized inside HeLa cells for cellular imaging. Additionally, the minima cytotoxic effect of PEGylated complexes in comparison to non-PEGylated samples was measured Rusing the WST-1 test and an In Cell Analyzer. A confocal microscopy study of the organelle morphology also confi rmed that the HeLa cell morphology was unchanged after treatment with PEGylated MWCs.

RSC Advances, 6(55), 49891-49902 (2016)

DOI: 10.1039/c6ra09191a   (Pobrane:  2018-04-04)


5.

Baranowska-Korczyc A., Warowicka A., Jasiurkowska-Delaporte M., Grześkowiak B., Jarek M., Maciejewska B.M., Jurga-Stopa J., Jurga S.

Antimicrobial electrospun poly(epsilon-caprolactone) scaffolds for gingival fibroblast growth This study discusses the value of polymer electrospun materials in three-dimensional (3D) scaffolds and antibacterial wound dressings for potential dental applications. Polycaprolactone (PCL) and polyvinylpyrrolidone (PVP) nanofibers were used as bases for gingival fibroblast (HGF-1 cell line) growth. HGF-1 cells cultured on both types of nanofibers were found to have normal morphology and growth by selective staining of the nuclei and cytoskeleton. The nanofibers were synthesized on different collectors to obtain a random or parallel alignment. Cell growth was observed along the nanofibers. In addition, antibiotics were incorporated within the nanofibers and studied by means of Raman spectroscopy and differential scanning calorimetry. The release profile of the antibiotics was determined by broad band dielectric measurements. The drug was found to be released by Fickian diffusion. The WST-1 test found PCL and PCL/ampicillin nanofibers to have minimal cytotoxicity. The antibacterial activity of materials containing ampicillin was evaluated by zone inhibition against a selected oral strain of Streptococcus sanguinis. The bacterial growth was inhibited by antibiotic release from PCL/ampicillin mats.

RSC Advances, 6(24), 19647-19656 (2016)

DOI: 10.1039/c6ra02486f   (Pobrane:  2018-04-03)


4.

Maciejewska B.M., Warowicka A., Baranowska-Korczyc A., Zaleski K., Zalewski T., Kozioł K.K., Jurga S.

Magnetic and hydrophilic MWCNT/Fe composites as potential T-2-weighted MRI contrast agents In this work, functionalized Multiwall Carbon Nanotubes of specified length and containing well-defined iron particles (O-MWCNT/Fe) were prepared. A significant enhancement in contrast in Magnetic Resonance Imaging was found for the investigated composites. The magnetic characterization revealed the ferromagnetic nature of iron particles embedded within O-MWCNTs. The enhancement of the H-1 spin-spin relaxation time of MRI scans using hydrophilic O-MWCNT/Fe as potential contrast agents was estimated for selected dispersive media. Moreover, the cytotoxicity of the hybrids was studied in two cell lines, i.e. cancer cells (HeLa) and fibroblasts (GM07492). The intracellular impact of O-MWCNT/Fes in HeLa cells was observed after staining of selected organelles (nuclei and mitochondria). Significant changes in cell morphology were found for water soluble MWCNT/Fes with diameters above 30 nm. (C) 2015 Elsevier Ltd. All rights reserved.

Carbon, 94, 1012-1020 (2015)

DOI: 10.1016/j.carbon.2015.07.091


3.

Maciejewska B.M., Coy L.E., Koziol K.K.K., Jurga S.

Facile synthesis of highly stable and water-soluble magnetic MWCNT/alpha-Fe nanocomposites Multiwall carbon nanotubes (MWCNT) were synthesized by the floating catalyst chemical vapor deposition (FCCVD) method. As a result, nanotubes containing metallic iron (a-Fe) were obtained and characterized. The impact of surface modification, on MWCNTs stability in water, was thoroughly studied applying three oxidative protocols. Samples were further characterized and correlated based on scanning electron microscopy (SEM), high resolution transmission electron microscopy (HR-TEM), Raman spectroscopy, Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), and thermal gravimetric analysis (TGA), and the magnetic nature of the embedded nanoparticles was assessed by means of a SQUID magnetometer at room temperature in powder. Finally, precise length segregation of MWCNT/alpha-Fe nanocomposites was achieved. The studied structures showed excellent quality and unmatched stability in water after more than three months.

The Journal of Physical Chemistry C, 118(4), 27861-27869 (2014)

DOI: 10.1021/jp5077142


2.

Maciejewska B.M., Jasiurkowska-Delaporte M., Vasylenko A.I., Kozioł K.K., Jurga S.

Experimental and theoretical studies on the mechanism for chemical oxidation of multiwalled carbon nanotubes In this study, the oxidation of multiwalled carbon nanotubes (MWCNTs) sonicated and/or refluxed in acids (H2SO4/HNO3) was investigated using a combination of high-resolution transmission electron microscopy, Raman spectroscopy, Fourier transform infrared spectroscopy, and ab initio computational methods. Sonication of the sample has the strongest effect, leading to the highest concentration of defects and carboxyl groups on the walls. Substantial correlations between treatment protocols, CNT size, and the types of chemical moieties are observed. Finally, based on experimental and computational results, we suggest the mechanism of the oxidation process for attaching the functional groups on the sidewalls.

RSC Advances, 4(55), 28826-28831 (2014)

DOI: 10.1039/c4ra03881a


1.

Soleimani H., Yahya N., Latiff N.R.A., Kozioł K., Maciejewska B.M., Guan B.H.

Synthesis and characterization of mesoporous multi-walled carbon nanotubes at low frequencies electromagnetic waves For electromagnetic absorbing and shielding applications, carbon nanotubes (CNT) are widely used due to their excellent electrical and physical properties. Fabrication of microwave absorbing materials involves the use of compounds capable of generating dielectric and/or magnetic losses when impinged by an electromagnetic wave. The presence of lattice defects e.g. vacancies and dislocations contributes to the loss and attenuation in the electromagnetic waves, which in turn remarkably enhance the absorption ability of the material. With the CVD technique which is known to produce several lattice defects in the final product, aligned MWCNTs were successfully synthesized by pyrolizing toluene and ferrocene in an inert argon environment. The morphology analysis of the aligned MWCNTs was conducted via FESEM and TEM analysis, to reveal the average length of approximately 295 mu m, with diameters in the range of 60-200 nm. EDS analysis indicates the high yield of CNTs, with more than 90% in weight composition, with less than 5 % Fe impurities presence. Textural properties of MWCNTs were studied by measuring pore size and BET surface area. To understand the response of CNTs to an electromagnetic field, permeability and permittivity measurement were conducted in the frequency range of 100 Hz to 110 MHz. In conclusion, the presence of defects in MWCNTs is desirable for enhanced electromagnetic absorption ability.

Journal of Nano Research, 26, 117-122 (2014)

DOI: 10.4028/www.scientific.net/JNanoR.26.117


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