Zakład Fizyki Makromolekularnej
Strona główna


od 2020-09-20

Dr inż. Martyna Michalska  | 2011-10 <> 2016-01

Stażysta podoktorski

   CNBM UAM   

  0000-0003-2910-2767     2303472     56002227400  

Publikacje                          Seminaria


Przysiecka Ł., Michalska M., Nowaczyk G., Peplińska B., Jesionowski T., Schneider R., Jurga S.

iRGD peptide as effective transporter of CuInZnxS2+x quantum dots into human cancer cells In this paper, iRGD peptide-mediated quantum dots (QDs) delivery was studied. In the first step, dodecanethiol-capped CulnZn(x)S(2+x) (ZCIS) QDs were prepared and subsequently transferred into water using a standard and facile ligand exchange approach involving 3-mercaptopropionic acid (MPA). ZCIS@MPA nanocrystals possess a photoluminescence quantum yield (PL QY) of 25%, a PL emission centered at ca. 640nm and low distributions in size and shape. Next, the iRGD peptide was electrostatically associated to ZCIS@MPA QDs. After cytotoxicity evaluation, the tumor-targeting and penetrating activities of the iRGD/QD assembly were investigated by confocal microscopy. The experiments performed on various cancer cell lines revealed a high penetration ability of the assembly, while the bare QDs were not internalized. Additionally, imaging experiments were conducted on three-dimensional multicellular tumor spheroids in order to mimic the tumor microenvironment in vivo. iRGD/QD assemblies were found to be evenly distributed throughout the whole HeLa spheroid contrary to normal cells where they were not present. Therefore, iRGD/QD assemblies have a great potential to be used as targeted imaging agents and/or nanocarriers specific to cancer cells.

Colloids and Surfaces B: Biointerfaces, 146, 9-18 (2016)

DOI: 10.1016/j.colsurfb.2016.05.041   (Pobrane:  2018-04-03)


Michalska M., Florczak A., Dams-Kozłowska H., Gapiński J., Jurga S., Schneider R.

Peptide-functionalized ZCIS QDs as fluorescent nanoprobe for targeted HER2-positive breast cancer cells imaging In this paper, the synthesis of alloyed CuInZnxS2+x quantum dots (ZCIS QDs), their transfer into aqueous solution via a polymer coating technique, and the use of these nanocrystals to selectively target HER2-positive cells, are reported. By optimizing first the ZnS shell deposition process onto the CuInS2 core, and next the encapsulation of the dots with the amphiphilic poly(maleic anhydride-alt-1-octadecene) (PMAO) polymer, water-dispersible ZCIS QDs were successfully prepared. The nanocrystals with a photoluminescence quantum yield of 35% were purified via centrifugation and ultracentrifugation and high quality nanoparticles with narrow size distributions and surface charges were obtained. After verifying the biocompatibility of PMO-coated ZCIS QDs, we coupled these nanocrystals with the LTVSPWY peptide and demonstrated via MTT assay that both bare and the peptide-linked QDs exhibit low cytotoxicity. The HER2-mediated delivery of the peptide-linked QDs was confirmed by confocal microscopy. This study indicates that as engineered QDs can efficiently be used as fluorescent nanoprobes for selective labelling of HER2-positive SKBR3 cancer cells. Copyright 2016 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.

Acta Biomaterialia, 35, 293-304 (2016)

DOI: 10.1016/j.actbio.2016.02.002   (Pobrane:  2018-04-05)


Michalska M., Aboulaich A., Medjahdi G., Mahiou R., Jurga S., Schneider R.

Amine ligands control of the optical properties and the shape of thermally grown core/shell CuInS2/ZnS quantum dots A series of CuInZnxS2+x QDs has been successfully synthesized via a non-injection method by varying the amine (oleylamine, dioctylamine, dodecylamine, octadecylamine) during the ZnS shell introduction on the CuInS2 core. The effects of these amines on the photoluminescence (PL) and on the morphology of CuInZnxS2+x QDs were explored. The optical features and structure of the obtained CuInZnxS2+x QDs have been characterized by UV visible and fluorescence spectroscopies, time resolved PL, HR-TEM, XRD and EDX. After the ZnS shell introduction at 220 degrees C, CuInZnxS2+x QDs showed greatly improved properties, the PL quantum yield can reach up to 70% using dioctylamine. The formation of CuInZnxS2+x QDs results in a blue shift of both the absorption and PL due to the formation of the alloyed interfacial layer at CuInS2/ZnS during the shell growth. By increasing the reaction temperature to 250 degrees C (thermolysis of CuInZnxS2+x QDs), marked red-shifts in PL emissions accompanied by morphological changes of the nanocrystals were observed. The use of oleylamine generates 6.6 nm-sized pyramidally-shaped nanocrystals with a PL quantum yield of 22%.
(C) 2015 Elsevier B.V. All rights reserved.

Journal of Alloys and Compunds, 645, 184-192 (2015)

DOI: 10.1016/j.jallcom.2015.04.162

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