Zakład Fizyki Makromolekularnej
Strona główna


od 2020-09-20

Dr Beata Wereszczyńska  | 2012-10 <> 2020-06


  0000-0003-3013-212X     56554233600  

Publikacje                          Seminaria


Maciejewska B.M., Wychowaniec J.K., Woźniak-Budych M., Popenda Ł., Warowicka A., Golba K., Litowczenko J., Fojud Z., Wereszczyńska B., Jurga S.

UV cross-linked polyvinylpyrrolidone electrospun fibres as antibacterial surfaces Many bacteria become progressively more resistant to antibiotics and it remains a challenging task to control their overall levels. Polymers combined with active biomolecules come to the forefront for the design of antibacterial materials that can address this encounter. In this work, we investigated the photo-crosslinking approach of UV-sensitive benzophenone molecule (BP) with polyvinylpyrrolidone (PVP) polymer within electrospun fibres. The BP and PVP solutions allowed fabricating polymer mats that were subsequently functionalised with antibacterial lysozyme. The physical properties of the crosslinked electrospun fibres were investigated by scanning electron microscopy and atomic force microscopy. The average diameter of the obtained fibres decreased from 290 ± 50 nm to 270 ± 70 nm upon the addition of the crosslinking molecules and then to 240 ± 80 nm and 180 ± 90 nm after subsequent crosslinking reaction at an increasing time: 3 and 5 h, respectively. The peak force quantitative nanomechanical mapping (PF-QNM) indicated the increase of DMT modulus of obtained cross-linked fibres from 4.1 ± 0.8 GPa to 7.2 ± 0.5 GPa. Furthermore, the successful crosslinking reaction of PVP and BP solution into hydrogels was investigated in terms of examining photo-crosslinking mechanism and was confirmed by rheology, Raman, Fourier transform infrared and nuclear magnetic resonance. Finally, lysozyme was successfully encapsulated within cross-linked PVP-BP hydrogels and these were successfully electrospun into mats which were found to be as effective antibacterial agents as pure lysozyme molecules. The dissolution rate of photo cross-linked PVP mats was observed to increase in comparison to pure PVP electrospun mats which opened a potential route for their use as antibacterial, on-demand, dissolvable coatings for various biomedical applications.

Science and Technology of Advanced Materials, 20(1), 979-991 (2019)

DOI: 10.1080/14686996.2019.1667737   (Pobrane:  2020-06-04)


Skupin-Mrugalska P., Sobotta Ł., Warowicka A., Wereszczyńska B., Zalewski T., Gierlich P., Jarek M., Nowaczyk G., Kempka M., Gapiński J., Jurga S., Mielcarek J.

Theranostic liposomes as a bimodal carrier for magnetic resonance imaging contrast agent and photosensitizer The present study is focused on the development of liposomes bearing gadolinium chelate (GdLip) providing two functionalities for magnetic resonance imaging (MRI) and photodynamic therapy of cancer. A lipid derivative of gadolinium(III) diethylenetriamine pentaacetic acid salt (GdDTPA1) was inserted in the liposomal membrane and served as MRI contrast agent whereas a zinc phthalocyanine (ZnPc) was used as a model photosensitizer. In addition to conventional liposomes, pegylated lipids were used for the preparation of "stealth" liposomes. The characterization of different GdLip formulations involved evaluation of the liposomes size by nanoparticle tracking analysis, thermal phase behavior by differential scanning calorimetry and ZnPc-mediated singlet oxygen production. Furthermore, relaxivity measurements were performed as well as cytotoxicity and photodynamic activity against cancerous and normal cell lines was studied. Size and thermal behavior were only slightly influenced by GdLip composition, however it distinctly affected singlet oxygen production of ZnPc-loaded GdLip. The quantum yields of singlet oxygen generation by zinc phthalocyanine incorporated in GdLip containing cationic or/and pegylated lipids were smaller than those obtained for non-pegylated carriers with l-alpha-phosphatidylglycerol. In general, all formulations of GdLip, irrespectively of composition, were characterized by relaxivities higher than those of commercially used contrast agents (e.g. Magnevist). NMR study has shown that the incorporation of ZnPc into the formulations of GdLip increases the relaxation parameters r1 and r2, compared to the values for the non-loaded vesicles. GdDTPA1 did not influence the photodynamic activity of ZnPc against HeLa cells. Copyright © 2017 Elsevier Inc. All rights reserved.

Journal of Inorganic Biochemistry, 180, 1-14 (2018)

DOI: 10.1016/j.jinorgbio.2017.11.025   (Pobrane:  2018-03-20)


Hałupka-Bryl M., Bednarowicz M., Dobosz B., Krzyminiewski R., Zalewski T., Wereszczyńska B., Nowaczyk G., Jarek M., Nagasaki Y.

Doxorubicin loaded PEG-b-poly(4-vinylbenzylphosphonate) coated magnetic iron oxide nanoparticles for targeted drug delivery Due to their unique physical properties, superparamagnetic iron oxide nanoparticles are increasingly used in medical applications. They are very useful carriers for delivering antitumor drugs in targeted cancer treatment. Magnetic nanoparticles with chemiotherapeutic were synthesized by coprecipitation method followed by coaling with biocompatible polymer. The aim of this work is to characterize physical and magnetic properties of synthesized nanoparicles. Characterization was carried out using EPR, HRTEM, X-ray diffraction, SQUID and NMR methods. The present findings show that synthesized nanosystem is promising tool for potential magnetic drug delivery.
(C) 2015 Elsevier B.V. All rights reserved

Journal of Magnetism and Magnetic Materials, 384, 320-327 (2015)

DOI: 10.1016/j.jmmm.2015.02.078   (Pobrane:  2020-10-23)

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