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wizyta

od 2020-09-20

Mgr Mikołaj Grzeszkowiak  | 2010-10 <> 2018-09

Doktorant

   CNBM UAM       grzeszkowiak.m@gmail.com


Publikacje                          Seminaria


9.

Matuła K., Richter Ł., Janczuk-Richter M., Nogala W., Grzeszkowiak M., Peplińska B., Jurga S., Wyroba E., Suski S., Bilski H., Silesian A., Bluyssen H.A.R., Derebecka N., Wesoły J., Łoś J.M., Łoś M., Decewicz P., Dziewit Ł., Paczesny J., Hołyst R.

Phenotypic plasticity of Escherichia Coli upon exposure to physical stress induced by ZnO nanorods Evolution of bacteria to selective chemical pressure (e.g. antibiotics) is well studied in contrast to the influence of physical stressors. Here we show that instantaneous physical stress in a homogeneous environment (without concentration gradient) induces fast adaptation of Escherichia coli. We exposed E. coli to a large number of collisions of around 10(5) per bacterium per second with sharp ZnO nanorods. The pressure exerted on the bacterial cell wall was up to 10 GPa and induced phenotype changes. The bacteria's shape became more spherical, the density of their periplasm increased by around 15% and the average thickness of the cell wall by 30%. Such E. coli cells appeared almost as Gram-positive bacteria in the standard Gram staining. Additionally, we observed a combination of changes occurring at the genomic level (mutations identified in form of single nucleotide polymorphisms) and downregulation of expression of 61 genes encoding proteins involved in beta-oxidation of fatty acids, glycolysis, the citric acid cycle, as well as uptake of amino acids and enzyme cofactors. Thus, we show that bacteria undergo phenotypic changes upon instantaneous, acute physical stress without any obviously available time for gradual adaptation.

Scientific Reports, 9, 8575 (2019)

DOI: 10.1038/s41598-019-44727-w   (Pobrane:  2020-12-30)


8.

Jenczyk J., Woźniak-Budych, M., Jarek M., Grzeszkowiak M., Nowaczyk G., Jurga S.

Nanoparticle string formation on self-assembled copolymer films Nanoparticles (NP) string formations on self-assembled copolymeric substrates has been observed. These "thread of beads" like structures develop via simple colloidal droplet evaporation during meniscus rim withdrawal on polystyrene-block-poly(ethylene oxide) (PS-PEO) copolymer surfaces. It is shown that the process is triggered by the presence of the substrate impurities, which lead to NP aggregate formations serving as string initiation sites. The growth mechanism of these linear structures seems to be capillarity-driven. Moreover, there is an exceptional alignment coupling between NP strips and the block copolymer (BC) domains observed. BC directed NP assembly stems from a gold nanocrystal surface functionalization, which introduces selective affinity for one particular type of BC domain. The presented results reveal a potential fabrication method of NP wires characterized by remarkably low width and thickness comparable with the size of the individual constituent NP.

Applied Surface Science, 406, 235-244 (2017)

DOI: 10.1016/j.apsusc.2017.02.143


7.

Grześkowiak B., Hryhorowicz M., Tuśnio K., Grzeszkowiak M., Zaleski K., Lipiński D., Zeyland J., Mykhaylyk Olga., Słomski R., Jurga S., Woźniak A.

Generation of transgenic porcine fibroblast cell lines using nanomagnetic gene delivery vectors The transgenic process allows for obtaining genetically modified animals for divers biomedical applications. A number of transgenic animals for xenotransplantation have been generated with the somatic cell nuclear transfer (SCNT) method. Thereby, efficient nucleic acid delivery to donor cells such as fibroblasts is of particular importance. The objective of this study was to establish stable transgene expressing porcine fetal fibroblast cell lines using magnetic nanoparticle-based gene delivery vectors under a gradient magnetic field. Magnetic transfection complexes prepared by self-assembly of suitable magnetic nanoparticles, plasmid DNA, and an enhancer under an inhomogeneous magnetic field enabled the rapid and efficient delivery of a gene construct (pCD59-GFPBsd) into porcine fetal fibroblasts. The applied vector dose was magnetically sedimented on the cell surface within 30min as visualized by fluorescence microscopy. The PCR and RT-PCR analysis confirmed not only the presence but also the expression of transgene in all magnetofected transgenic fibroblast cell lines which survived antibiotic selection. The cells were characterized by high survival rates and proliferative activities as well as correct chromosome number. The developed nanomagnetic gene delivery formulation proved to be an effective tool for the production of genetically engineered fibroblasts and may be used in future in SCNT techniques for breeding new transgenic animals for the purpose of xenotransplantation.

Molecular Biotechnology, 58(5), 351-61 (2016)

DOI: 10.1007/s12033-016-9934-1


6.

Kiwilsza A., Milanowski B., Drużbicki K., Coy L.E., Grzeszkowiak M., Jarek M., Mielcarek J., Lulek J., Pajzderska A., Wąsicki J.

Mesoporous drug carrier systems for enhanced delivery rate of poorly water-soluble drug: nimodipine Two mesoporous silica materials: MCM-41 and SBA-15 were applied as potential nanocarriers for poorly soluble drug—nimodipine. Drug incorporation was performed using modified adsorption from the solution method and loaded samples before and after washing procedure were studied. The physical properties were verified by: differential scanning calorimetry, X-ray powder diffraction, electron microscopies (SEM/TEM) and Fourier-transform infrared spectroscopy (FT-IR). FT-IR results for bulk nimodipine were interpreted on the basis of first principles calculations (DFT). As a result of encapsulation process, in both matrices nimodipine appeared simultaneously in two forms: crystalline and amorphous, but the first one turned out to be easily removable during washing procedure. The in vitro dissolution and release tests were performed with ultra pure water under supersaturating conditions. The release rate of the amorphous nimodipine from mesoporous silica materials was at least 70 times higher than dissolution rate of bulk drug, thus revealed a potential usefulness of such carrier in future pharmaceutical applications in terms of delivery of poorly soluble drugs.

Journal of Porous Materials, 22(3), 817-829 (2015)

DOI: 10.1007/s10934-015-9955-3   (Pobrane:  2020-10-23)


5.

Jeżowski P., Nowicki M., Grzeszkowiak M., Czajka R., Beguin F.

Chemical etching of stainless steel 301 for improving performance of electrochemical capacitors in aqueous electrolyte The main purpose of the study was to increase the surface roughness of stainless steel 301 current collectors by etching, in order to improve the electrochemical performance of electrical double-layer capacitors (EDLC) in 1 mol L−1 lithium sulphate electrolyte. Etching was realized in 1:3:30 (HNO3:HCl:H2O) solution with times varying up to 10 min. For the considered 15 μm thick foil and a mass loss around 0.4 wt.%, pitting was uniform, with diameter of pits ranging from 100 to 300 nm. Atomic force microscopy (AFM) showed an increase of average surface roughness (Ra) from 5 nm for the as-received stainless steel foil to 24 nm for the pitted material. Electrochemical impedance spectroscopy realized on EDLCs with coated electrodes either on as-received or pitted foil in 1 mol L−1 Li2SO4 gave equivalent distributed resistance (EDR) of 8 Ω and 2 Ω, respectively, demonstrating a substantial improvement of collector/electrode interface after pitting. Correlatively, the EDLCs with pitted collector displayed a better charge propagation and low ohmic losses even at relatively high current of 20 A g−1. Hence, chemical pitting of stainless steel current collectors is an appropriate method for optimising the performance of EDLCs in neutral aqueous electrolyte

Journal of Power Sources, 279, 555-562 (2015)

DOI: 10.1016/j.jpowsour.2015.01.027


4.

Różycka M., Wojtas M., Jakób M., Stigloher Ch., Grzeszkowiak M., Mazur M.

PLoS ONE, 10(3), e0119969 (2015)

DOI: 10.1371/journal.pone.0119969


3.

Deptuła T., Warowicka A., Woźniak A., Grzeszkowiak M., Jarzębski M., Bednarowicz M., Patkowski A., Słomski R.

Cytotoxicity of thermo-responsive polymeric nanoparticles based on N-isopropylacrylamide for potential application as a bioscaffold Polymeric nanoparticles based on poly-N-isopropylacrylamide (pNiPAM NPs) and their bio-medical applications have been widely investigated in recent years. These tunable nanoparticles are considered to be great candidates for drug delivery systems, biosensors and bioanalytical devices. Thus, the biocompatibility and toxicity of these nanoparticles is clearly a crucial issue. In this work, the cytotoxicity of thermo-responsive pNiPAM nanoparticles was studied, followed by a detailed analysis of the NPs morphology in growing cell cultures and their 3D structure. Cytotoxic examination was conducted for two cell cultures - HeLa (cervical cancer cell line) and HeK293 (human embryonic kidney cell line), employing MTT (3-4, 5-dimethylthiazol-2-yl-2, 5-diphenyltetrazolium bromide) assay and viability tests. We used Cryo-SEM (scanning electron microscopy) and fluorescence microscopy (IN Cell Analyzer) in order to investigate the morphological structure of the polymer network. We show that pNiPAM nanoparticles do not exhibit any cytotoxicity effects on the investigated cell lines. Additionally, we report that the pNiPAM nanoparticle based scaffold promotes cell growth.

Acta Biochimica Polonica, 62(2), 311-316 (2015)

DOI: 10.18388/abp.2015_1007


2.

Różycka M., Wojtas M., Jakób M., Stigloher Ch., Grzeszkowiak M., Mazur M., Ożyhar A.

Intrinsically Disordered and Pliable Starmaker-Like Protein from Medaka (Oryzias latipes) Controls the Formation of Calcium Carbonate Crystals Fish otoliths, biominerals composed of calcium carbonate with a small amount of organic matrix, are involved in the functioning of the inner ear. Starmaker (Stm) from zebrafish (Danio rerio) was the first protein found to be capable of controlling the formation of otoliths. Recently, a gene was identified encoding the Starmaker-like (Stm-l) protein from medaka (Oryzias latipes), a putative homologue of Stm and human dentine sialophosphoprotein. Although there is no sequence similarity between Stm-l and Stm, Stm-l was suggested to be involved in the biomineralization of otoliths, as had been observed for Stm even before. The molecular properties and functioning of Stm-l as a putative regulatory protein in otolith formation have not been characterized yet. A comprehensive biochemical and biophysical analysis of recombinant Stm-l, along with in silico examinations, indicated that Stm-l exhibits properties of a coil-like intrinsically disordered protein. Stm-l possesses an elongated and pliable structure that is able to adopt a more ordered and rigid conformation under the influence of different factors. An in vitro assay of the biomineralization activity of Stm-l indicated that Stm-l affected the size, shape and number of calcium carbonate crystals. The functional significance of intrinsically disordered properties of Stm-l and the possible role of this protein in controlling the formation of calcium carbonate crystals is discussed.

PLoS ONE, 9(12), (2014)

DOI: 10.1371/journal.pone.0114308


1.

Śliwa T., Jarzębski M., Gapiński J., Grzeszkowiak M., Kleshchanok D.

Acta Physica Polonica A, 125(5), 1236-1239 (2014)

DOI: 10.12693/APhysPolA.125.1236


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