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wizyta

od 2020-09-20

Mgr Joanna Wolak  | 2017-10 - obecnie

Doktorant

   WF UAM pokój: 132      +48 61-829-5238       joanna.wolak@amu.edu.pl

  0000-0001-5656-1737     4233810 / AAE-5481-2021     57219639265  

Publikacje                          Seminaria


2.

Wolak J., Skupin M., Pietralik Z., Andrzejewska W., Kozak M.

Abstrakt lub materiały konferencyjne, albo abstrakt publikacji
z dodatkowymi numerami DOI

Studies of zwitterionic lipoplexes - nanosystem based on phospholipids and surfactants as innovative delivery systems for gene therapy The gene therapy is the one of the most promising method of treatment in contemporary medicine. This way of therapy is very useful in the treatment a dozen of incurable or fatal diseases. This method of treatment is leaned on implement a gene into patient's cells with the use of dedicated delivery systems (vectors). The main problem of gene therapy is to find the best vector which will be effective and will be not toxic for human cells. A good approach seems to be use of non-viral vectors like delivery system based on lipid-surfactant mixtures.

The aim of this study was to examine the possible application of selected amphoteric surfactants (zwitterionic alkyl derivatives of sulfobetaine) as complexing agents (with and without helper lipid) for nucleic acids (siRNA, low and high-molecular weight DNA).

The studies of DNA conformation in selected DNA - zwitterionic surfactant lipoplexes were performed using the circular dichroism (CD) spectroscopy. CD spectra were recorded in the spectral range 350 - 200 nm by using J-815 spectrometer (Jasco). The results obtained indicate that the DNA maintains the B-form for wide range of surfactant concentrations in the solution. The structure and organization of lipoplexes was also independently analyzed by the Fourier transform infrared spectroscopy. The absorption spectra for lipoplexes were collected by using FTIR spectrometer BRUKER Tensor 27 in the temperature range 2-40°C. The phase transitions in examined systems were studied by using the differential scanning calorimetry (DSC). Ability of creating of stable complexes in DNA-surfactant systems studied was confirmed using electrophoresis on agarose gel. For all formed stable lipoplexes the complete reduction of electrophoretic mobility was observed. Finally the transfection efficiency of selected systems were also tested on HeLa cells.

Biophysical Journal, 108(2) S1, 545A (2015)

DOI: 10.1016/j.bpj.2014.11.2989   (Pobrane:  2020-11-05)


1.

Skupin M., Wolak J., Kozak M.

Abstrakt lub materiały konferencyjne, albo abstrakt publikacji
z dodatkowymi numerami DOI

Nanosystem based on phospholipids and surfactants as innovative delivery system for gene therapy Amphiphilic dicationic surfactants, known as gemini surfactants, are currently studied for gene delivery purposes. The biggest advantages of these systems are that they are non-immunogenic and generally have low toxicity. One of the most important advantages of these systems is improved transfection efficiency. The aim of this study was to determine the possibility to use amphoteric surfactants (zwitterionic derivatives of sulfobetaine with carbohydrate moiety) and sulfobetaine/gemini surfactant mixtures as complexing agents for nucleic acids, with potential applications for gene delivery to reduce the toxicity and improved transfection. Fourier transform infrared (FTIR) spectroscopy and differential scanning calorimetry (DSC) were used to a7nalyze influence of surfactants on the phase behaviour of 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) bilayers with the presence of different DNA forms (small DNA oligomers, cDNA, low and high-molecular mass DNA). The influence of different concentrations of sulfobetaine and sulfobetaine/gemini surfactant mixtures with the presence of DNA on creating stable complexes was investigated using circular dichroism (CD) spectroscopy and electrophoresis. A series of measurements of toxicity and transfection of these lipoplexes were performed in HeLa cells. These compounds appear to be excellent for creating complexes with DNA. Thanks to their construction this DNA carrier molecules might be able to deliver genes to the cells of almost any DNA mo7lecular size, unattainable when using viral gene delivery systems.

Biophysical Journal, 108(2) S1, 245A (2015)

DOI: 10.1016/j.bpj.2014.11.1357   (Pobrane:  2020-08-10)


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